Preparing glands for SEM imaging – making pelts of glands. Potassium hydroxide digestion leaves behind the sclerotized gland reservoir and ducts, while removing unwanted soft tissue.
An image showing a concentration of D1 gland cell ducts.
Developing abdominal defensive gland in a 48 hour old Atheta coriaria pupa. Already a dense outline of type 1, D2 epidermal secretory cells are visible outlining the glandular reservoir, which itself is derived from the intersegmental membrane between tergites VI and VII. Pupal duration is approximately 96 hours long in this species.
The arrow indicates the cell-dense intersegmental invagination which is the defensive gland reservoir.
And another view.
These images were taken with the help of entomology Steve. Thin sections were taken in epoxy embedded samples, then colored in a general stain – hence the blue. Thanks Steve!
Big fan of strong, attitude-pumped female vocal-centric groups, not to mentioned, I’m kinda into electro-pop right now. As I have, please totally rock out as you carefully contemplate the gland images as a representation of a key innovation – a key innovation that compartmentalized bodily glandular tissue into a collective organ, helps natural selection do it’s thing this way, at least that’s my working hypothesis. I said it first, just saying.
My mom sent me this mural on a school wall, somewhere in Japan.
Yuu clearly has a shady dad that she enjoys hanging out with; Yuika likes new dresses, but unfortunately is vomiting all over this new one form too many strawberry daiquiri; Takemi has no artistic skills what so ever, but none of us expect much from him; Yuuta enjoys soccer with his leg-less brother; Mako can double-fist any cone; Mayo has diva written all over her, but a pair of legs would really help with the dancing in singing and dancing.
But wait, what is this, Taro draws a ladybug?! Whaaaat?! Parents, if you think a name has no bearing on a childs future as a poor entomologist, think again. He’ll eventually turn to smaller insects people care even less about, don’t worry there folks.
On another note, I’ve updated my website to include a local (near Lawrence, KS) Trichopsenius sp. A beetle that lives with Reticulitermes termites. This one is post-extraction, not bad. I’m going to try this on a more mushy-mushy (no pun intended, for those that get it) physogastric Xenistusa and see how I fare.
I’m also super into ~90′s feminist punk/electro pop, spawns of the Riot Grrrl movement. Here’s Le Tigre, a Bikini Kill offshoot.